《植物生理学报》 2009, 45(3): 239-243

特矮稻ED 基因的遗传定位和分析

梅进¹, 杨远柱^2, 林建中¹, 胡小淳², 周波¹, 赵小英¹, 符辰建², 黄星群¹, 唐冬英¹, 刘选明^1,*

1 湖南大学生命科学与技术研究院, 生物能源与材料研究中心, 长沙410082; 2 湖南亚华种业科学研究院, 长沙410119

通信作者：刘选明；E-mail: sw_xml@hnu.cn；Tel: 0731-8821721

摘　要：

赤霉素(GA3)鉴定表明, 一种新型特矮稻(命名为‘ 特矮稻-2’)的GA3 信号转导途径正常, 施加外源GA3 不能恢复到正常植株的高度, 说明此种矮稻的矮化机制与GA3 无关。来源于组合‘ 特矮稻-2’ב 日本晴’ 的F2 群体的遗传分析表明, ‘ 特矮稻-2’ 的特矮性状受1 对隐性基因控制。采用SSR分子标记, 将该矮秆基因定位于第12 染色体上的RM519和RM235标记之间, 遗传距离分别为15.9 和22.0 cM, 该基因暂命名为ED。

关键词：水稻; GA3鉴定; 矮秆基因; 遗传分析; 基因定位

收稿：2008-11-13   修定：2009-02-10

资助：高等学校国家“985 工程” 科技创新平台项目(20005)。

Characterization and Genetic Mapping of ED from a Novel Dwarfing Rice (Oryza sativa L.)

MEI Jin¹, YANG Yuan-Zhu², LIN Jian-Zhong¹, HU Xiao-Chun², ZHOU Bo¹, ZHAO Xiao-Ying¹, FU Chen-Jian², HUANG XingQun¹, TANG Dong-Ying¹, LIU Xuan-Ming^1,*

¹Bioenergy and Biomaterial Research Center, Institute of Bioscience and Biotechnology, Hunan University, Changsha 410082, China; ²Academy of Seed Industry of Hunan Yahua, Changsha 410119, China

Corresponding author: LIU Xuan-Ming; E-mail: sw_xml@hnu.cn; Tel: 0731-8821721

Abstract:

We investigated gibberellin (GA₃) signaling in a novel dwarfing rice (Oryza sativa) ‘Teaidao-2’, the results showed that exogenous GA₃ could not rescue the dwarfing phenotype of ‘Teaidao-2’. So GA₃ was not involved in the dwarfing phenotype. The dwarfing gene was referred to as ED, a F₂ population derived from cross between ‘Teaidao-2’ and ‘Nipponbare’ was constructed for mapping of the ED locus. The dwarfing phenotype of ‘Teaidao-2’ was regulated by a single recessive gene. Simple sequence repeat (SSR) marker analyses revealed that ED was located between RM519 and RM235 with the genetic distance of 15.9 and 22.0 cM on chromosome 12.

Key words: rice (Oryza sativa); GA₃ identification; dwarfing gene; genetic analysis; gene mapping